Hrbek, T., Taphorn, DC and JE Thomerson (2005) - Molecular phylogeny of Austrofundulus Myers (Cyprinodontiformes: Rivulidae), with revision of the genus and the description of four new species. Zootaxa, 825: 1-39. ABSTRACT Phylogenetic analysis of 13 mitochondrial DNA genes of Austrofundulus Myers 1932 indicates that as presently recognized, A. limnaeus is composed of several populations with monophyletic haplotype lineages, which together are paraphyletic with respected to A. transilis. These populations were previously united based on shared plesiomorphic morphometric characters. Austrofundulus myersi is removed from synonymy; four new species: A. rupununi, A. leohoignei, A. guajira, and A. leoni are described; and A. limnaeus is restricted to populations along the eastern side of Lake Maracaibo. In contrast, populations of A. transilis from the Río Apure Llanos and the lower Río Unare basin show little divergence. The proposed phylogeny: (A. myersi (A. leoni (A. limnaeus (A. guajira (A. leohoignei (A. rupununi (A. transilis))))))) is strongly supported by high bootstrap values and Bremer decay indexes, and unique length variations in the 12S ribosomal DNA (rDNA) gene. The distributions and the relationships of the newly erected species are concordant with the geological history of northern South America... Abstract and excerpt available here http://www.mapress.com/zootaxa/2005f/z00825f.pdf on Zootaxa